smallRNA / miRNA Seq Analysis

Low amounts,  short turnaround time, PCR bias elimination

We use our gel-free single tube protocol for high quality smallRNA library preparation. Our technology (sample preparation and “TrueQuant“) effectively reduces ligation-bias and PCR-bias observed in other protocols. Our TrueQuant PCR bias elimnation is especially useful for smallRNA analysis, as the number of different molecules is limited.

Only 1 ng of total RNA is required as input material for our smallRNA Seq service.

Our know how about smallRNA library preparation was also integrated into our smallRNA-Seq kit: the only gel-free single-tube protocol for less then 10 ng of total RNA input material!

Bioinformatics

  • Annotation to existing databases (e.g.miRBase)
  • Interaction network analysis of miRNAs and target transcripts (optional)

Required Material: 1 µg of total RNA including microRNAs (please inquire for appropriate protocol) or appropriate amount of sample tissue

Low amountsof input material ?

We have developed an optimzed protocol for low amounts of material. Because we can eliminate PCR-bias thanks to our “TrueQuant” method, the data remains more accurate even at higher numbers of PCR cycles.

Required material:  1-100 ng of total RNA or the corresponding amount of fresh material. Please contact us for further information at info@genxpro.de or give us a call at +49 69 95739710.

qPCR

besides NGS-based smallRNA Analysis we also offer qPCR solutions for smallRNA.

miRNA_effect