smallRNA / miRNA Seq Analysis
Low amounts, short turnaround time, PCR bias elimination
We use our gel-free single tube protocol for high quality smallRNA library preparation. Our technology (sample preparation and “TrueQuant“) effectively reduces ligation-bias and PCR-bias observed in other protocols. Our TrueQuant PCR bias elimnation is especially useful for smallRNA analysis, as the number of different molecules is limited.
Only 1 ng of total RNA is required as input material for our smallRNA Seq service.
Our know how about smallRNA library preparation was also integrated into our smallRNA-Seq kit: the only gel-free single-tube protocol for less then 10 ng of total RNA input material!
- Annotation to existing databases (e.g.miRBase)
- Quantification (“TrueQuant“)
- Interaction network analysis of miRNAs and target transcripts (optional)
Required Material: 1 µg of total RNA including microRNAs (please inquire for appropriate protocol) or appropriate amount of sample tissue
Low amountsof input material ?
We have developed an optimzed protocol for low amounts of material. Because we can eliminate PCR-bias thanks to our “TrueQuant” method, the data remains more accurate even at higher numbers of PCR cycles.
Required material: 1-100 ng of total RNA or the corresponding amount of fresh material. Please contact us for further information at email@example.com or give us a call at +49 69 95739710.
besides NGS-based smallRNA Analysis we also offer qPCR solutions for smallRNA.