TrueQuant SmallRNA Seq Kit

The GenXPro small RNA sequencing kit (v1.0) can be used to prepare small RNA libraries to be sequenced on Illumina® NGS instruments starting with 0.1 to 500 ng of total RNA or down to 0.01 ng purified small RNA.

The library preparation methodology used in this kit allows an accurate identification and quantification of microRNAs, piRNAs and other small RNAs.

Simple single Tube Protocol, Gel free!

The entire workflow is occuring in a single tube. This minimizes losses and increases the efficiency tremendously.  Even 0.01 ng of total RNA can be sufficient input material for library preparation.

TrueQuant Technology

GenXPro small RNA sequencing kit applies the proprietary TrueQuant Technology that eliminates PCR-derived copies from the generated gene expression data. Following sequencing of the adaptor-ligated and PCR-amplified libraries, only the sequence with highest quality of each TrueQuant adapter plus template-sequence combination are maintained.

Liquid Biopsies from only 200 µl of Plasma

our protocol allows to generate smallRNA-Seq profiles from down to 200 µl of plasma.

Protocol for low input amounts, even from strongly degraded total RNA

Because of the single tube protocol that avoids gel purification steps,  combined with the TrueQuant method, very low amounts of input material (0.01 ng) even from strongly degraded total RNA is suitable for processing.

Principle and Procedure

Starting with DNase-treated and quality-controlled total RNA, adapters containint ght TrueQuant barcodes are ligated to both ends of the smallRNA, reverse transcribed and the final libraries are ready for sequencing on any of the Illumina MiniSeq, MiSeq, HiSeq, and NextSeq platforms. The present kit allows for multiplexing of up to 8 samples.

Free trial !

Please send us an e-mail (info@genxpro.de)  if you are interested in a free trial of our smallRNA-kit.

Service:

we also offer regular and ultra-low input smallRNA seq service.

Price:

Kit for MiSeq,  HiSeq or NextSeq (Illumina), Single End Sequencing

6 x Kit:                                                             540,00 EUR (cat. no. 16047.1)

Bioinformatics voucher 6 x:                        240,00 EUR (cat. no.16047.2 )

24 x Kit:                                                         1950,00 EUR (cat. no. 16047.3)

Bioinformatics voucher 24 x:                      768,00 EUR (cat. no.16047.4)

For orders or questions, please contact: info@genxpro.de

References:

Wecker T, Hoffmeier K, Plötner A, Grüning BA, Horres R, Backofen R, Reinhard T, Schlunck G.MicroRNA Profiling in Aqueous Humor of Individual Human Eyes by Next-Generation Sequencing.Invest Ophthalmol Vis Sci. 2016 Apr 1;57(4):1706-1713.

Müller S, Raulefs S, Bruns P, Afonso-Grunz F, Plötner A, Thermann R, Jäger C, Schlitter AM, Kong B, Regel I, Roth WK, Rotter B, Hoffmeier K, Kahl G, Koch I, Theis FJ, Kleeff J, Winter P, Michalski CW. Next-generation sequencing reveals novel differentially regulated mRNAs, lncRNAs, miRNAs, sdRNAs and a piRNA in pancreatic cancer. Mol Cancer. 2015 Apr 25;14(1):94.

From tissue to results- full service or kits for all your samples