High-Sensitivity NGS Library Prep Kit for cfDNA and DNA
Unlock ultrasensitive sequencing with UMIs – for liquid biopsies, tissue, cellular DNA and non-model organisms
Our kit for NGS library preparation of circulating free DNA (cfDNA) from blood plasma or serum (“Liquid Biopsy”) includes dual indices, TrueQuant-Molecular Barcodes (UMIs) and allows to generate NGS libraries from only 1ng of cfDNA.
Cell-free DNA, also referred to as circulating- free DNA (cfDNA) or circulating-cell-free DNA (ccfDNA), or as circulating-tumor DNA (ctDNA) when derived from tumors, is a powerful source of biomarkers used in liquid biopsies.
Key Features
- High sensitivity down to 1 ng input for cfDNA.
- Includes dual indexing and TrueQuant molecular barcodes (UMIs) for error correction, PCR bias reduction, and accurate detection of low-frequency variants.
- Single-tube workflow for ease of use.
- Compatible with high-throughput formats (dual index, up to 384 samples in parallel).
The kits can be applied for the analyses of circulating tumor DNA (ctDNA) in plasma or urine (liquid biopsies) or for NIPT and other applications.
Workflow
Adapters Versions
The adapters contain either a UMI at the place of the P7 ID (V2) or an additional UMI in front of the P7-ID (V2).
cfDNA Sequencing Service
We offer comprehensive bioinformatics support for model and non-model organisms, including assembly, annotation, SNP-detection etc. For human DNA, a detailed report including features such as Single Nucleotide Polymorphisms (SNPs), Fusions, Copy Number Variations (CNVs), Microsatellite Instability (MSI), HR (Homologous Recombination) is generated and provides connections to numerous databases (CIVIC, ClinVar, dbSNP, and more).
Ask for a free trial kit
Please send us an e-mail at info@genxpro.de or give us a call at: +49 69 95739710
Disclaimer
The kit is for research use only.
