Ultra low input,  avoidance of ligation- and PCR-bias due to patented UMI-technique, highest diversity of different RNA molecules

• We use our gel-free single tube protocol for high quality smallRNA library preparation.
• Our technology (sample preparation and “TrueQuant“) effectively reduces ligation-bias and PCR-bias observed in other protocols.
• TrueQuant PCR bias elimination is especially useful for smallRNA analysis, as the number of different molecules is limited.
• Our highly efficient library generation procedure effectively reduces the number  of PCR cylces and thereby reduced the number of duplicates
• The method works for all sources of smallRNA, including from FFPE, plasma, Exosomal Vesicles (EVs), urine, saliva, aqueous humor,  plants and other organisms

Workflow

Bioinformatics

• Annotation to existing databases (e.g.miRBase)
• Quantification (“TrueQuant“)
• Interaction network analysis of miRNAs and target transcripts (optional)

Ultra low input!

We have developed an optimzed protocol for low amounts of material. Because we can eliminate PCR-bias thanks to our “TrueQuant” method, the data remains more accurate even at higher numbers of PCR cycles.

Required material: 0.1-100 ng of total RNA or the corresponding amount of fresh material. Please contact us for further information at info@genxpro.de or give us a call at +49 69 95739710.

Liquid biopsies / exRNA / plasma or serum

because of its high sensitivity and the fact that our method works already with minute amounts of input material, only 25 µl of blood plasma is sufficient for the analyses of free circulating RNA or exRNA from blood plasma. The method is equally suited for the analyses of exRNA from urine or even aqueous humor.

Diversity of RNA species  – Comparison

SmallRNA yield from different kits using 200 ng of total RNA as input material- GenXPro method with highest diversity of different RNA molecules: from all different RNA-species, our TrueQuant smallRNA-Seq Kit missed the lowest number of different RNA-species, below depicted as “missed RNA mi, – t or other RNAs”.

CRISPR guide RNA (gRNA / sgRNA) quality control

Our TrueQuant smallRNA kit is ideally suited for quality control of CRISPR guideRNA (gRNA) and of other RNA constructs including miRNAs or siRNA with a size of up to 100 bps. We also provide powerful solutions for the quality control of longer RNA such as mRNA constructs. For DNA-constructs such as aptamers or plasmids please see https://genxpro.net/aptamers/.

Profit from our well-established nucleic-acid quality control (“NAQC”) analysis pipeline to quantify precisely all variants of the constructs to avoid off-target effects.

We offer the CRISPR guide RNA QC as full service or you can use our TrueQuant smallRNA seq kit and upload the data to our NAQC pipeline for bioinformatics analyses.

qPCR

Besides NGS-based smallRNA analysis, we also offer qPCR solutions for smallRNA.

smallRNA-Seq Kit

Our “know how” about smallRNA library preparation was also integrated into our smallRNA-Seq kit: the only gel-free single-tube protocol for less than 0.1 ng of total RNA input material.

Recent Publication

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From tissue to results- full service or kits for all your samples

MACE kit
Gel Free smallRNASeq kit