smallRNA / miRNA Seq Analysis Service
Low amounts, short turnaround time, PCR bias elimination
We use our gel-free single tube protocol for high quality smallRNA library preparation. Our technology (sample preparation and “TrueQuant“) effectively reduces ligation-bias and PCR-bias observed in other protocols. Our TrueQuant PCR bias elimnation is especially useful for smallRNA analysis, as the number of different molecules is limited.
Only 0.1 ng of total RNA is required as input material for our smallRNA Seq service.
- Annotation to existing databases (e.g.miRBase)
- Quantification (“TrueQuant“)
- Interaction network analysis of miRNAs and target transcripts (optional)
Required Material: 1 µg of total RNA including microRNAs (please inquire for appropriate protocol) or appropriate amount of sample tissue
Low amounts of input material ?
We have developed an optimzed protocol for low amounts of material. Because we can eliminate PCR-bias thanks to our “TrueQuant” method, the data remains more accurate even at higher numbers of PCR cycles.
Required material: 0.1-100 ng of total RNA or the corresponding amount of fresh material. Please contact us for further information at firstname.lastname@example.org or give us a call at +49 69 95739710.
besides NGS-based smallRNA Analysis we also offer qPCR solutions for smallRNA.
Our know how about smallRNA library preparation was also integrated into our smallRNA-Seq kit: the only gel-free single-tube protocol for less than 0.1 ng of total RNA input material.
From tissue to results- full service or kits for all your samples
Gel Free smallRNASeq kit