The MACE-Seq Kit
High Resolution Gene Expression at only 10% of RNA-Seq costs
Our highly successful MACE-Seq service is now also available as a kit. It contains all ingredients to perform MACE-Seq. In contrast to other “3’mRNA Seq” or “Tag-Seq” approaches the MACE-Seq Kit ncludes our patented TrueQuant method (molecular barcodes) to avoid PCR-bias, for the generation of qualitative and quantitative accurate data on any Illumina sequening machine. MACE-Seq produces allows for accurat end cost efficient high-resolution gene expression profiling, genotyping in transcripts, allele frequency determination and the analysis of alternative polyadenylation. Up to 96 samples can be processed in parallel.
The MACE kit comes with bioinformatics-vouchers for a convenient analysis of the data on our servers. As MACE data have some specificities, we have developed an optimized analysis pipeline that you can access using the vouchers. Simply upload your data using the provided voucher-codes to our servers. We provide complete result-tables compatible with Excel as well as plots of your data and Gene Ontology enrichment analyses. The raw and the refined data is accessible via our easily understandable web interface at http://tools.genxpro.net.
Further bioinformatics analyses are possible e.g. SNP-analysis, alternative polyadenylation or allele frequency determination (see Bioinformatics)
Therefore you do not need bioinformatians nor special hardware for the detailed analysis of the complex data.
Analyse 6 samples simultaneously on the MiSeq or 96 samples on a HiSeq or NextSeq500
The kit is available as 6x MACE version for up to 6 MACE libraries on the Illumina MiSeq platform and a 24x version for the Illumina HiSeq or NextSeq.
With further indices, several kits can also be combined for example to reach 4×24 samples.
Required input material: >10 ng totalRNA (can vary depending on tissue and quality of RNA)
Number of samples 1 – 96 (depending on Kit)
Time for sample preparation of 96 samples: 2 days
Kit for MiSeq (Illumina), Single End Sequencing
6x Kit: 390.00 EUR (cat. no. 16046.1)
Bioinformatics voucher 6x: 360.00 EUR (cat. no.16046.2)
Kit for HiSeq or NextSeq (Illumina), Single End Sequencing
24x Kit: 1,320.00 EUR (cat. no.160424.1)
2x Additional Post-Pool reactions1: 80.00 EUR (cat.no. 16040.1)
4x Additional Post-Pool reactions1: 140.00 EUR (cat.no. 16040.2)
Bioinformatics voucher 24x*: 1,080.00 EUR (cat. no.160424.2)
Additional adapter set to combine up to four kits: 160.00 EUR (cat. no.160424.3)
(All prices without tax)
*the bioinformatics is limited to model organisms and includes annotation, quantification, differential gene expression (DE) analyses of pairwise comparisons (p-values for DE, log 2 Fold Changes) and GO enrichment analyses in our MACE cloud server. Data is accessible as downloadble .xls table and through our web interface at http://tools.genxpro.net
1Pooling strategy: One advantage of the MACE kit is that the samples are pooled after generating barcoded cDNAs. The 6x and 24x kits are designed to pool up to 6 and 24 libraries, respectively. The kit contains reactions to generate up to 6 or 24 libraries in the pre-pooling step and one post-pool reaction in the 6x kit and two post pool reactions in the 24x kit. For pools below 6 samples (e.g. for only two samples), additional post-pooling reagents can be purchased separately.
Please send us an e-mail at firstname.lastname@example.org or give us a call at: +49 69 95739710
Please contact us for a free trial kit at email@example.com
Customers in Japan: please contact FUNAKOSHI.
NEWS: Our kit receieved five Stars from “BIOZ“
We also offer MACE as a service including optional RNA extraction and bioinformatics.
The MACE-kit is for research use only.