The MACE-Seq Kit
Our highly successful MACE-Seq service is now also available as a kit. It contains all ingredients to perform MACE-Seq. In contrast to other “3’mRNA Seq” or “Tag-Seq” approaches the MACE-Seq Kit includes our patented TrueQuant method (molecular barcodes; Unique Molecular Identifiers, UMIs) to avoid PCR-bias, for the generation of qualitative and quantitative accurate data on any Illumina sequencing machine. MACE-Seq allows for accurate and cost efficient high-resolution gene expression profiling, genotyping in transcripts, allele frequency determination and the analysis of alternative polyadenylation. Up to 386 samples can be processed in parallel.
The MACE kit comes with optional bioinformatics-vouchers for a convenient analysis of the data on our servers. As MACE data have some specificities, we have developed an optimized analysis pipeline that you can access using the vouchers. Simply upload your data using the provided voucher-codes to our servers. We provide complete result tables compatible with Excel, as well as plots of your data and Gene Ontology enrichment analyses. The raw and the refined data is accessible via our easily understandable web interface at http://tools.genxpro.net.
Further bioinformatics analyses are possible e.g. SNP-analysis, alternative polyadenylation or allele frequency determination (see Bioinformatics).
Therefore you do not need bioinformatians nor special hardware for the detailed analysis of the complex data.
Analyse 6 samples simultaneously on the MiSeq or 96-384 samples on a HiSeq, NextSeq500 or NovaSeq
The kit is available as 6x MACE version for up to 6 MACE libraries on the Illumina MiSeq platform and a 24-48x version for the Illumina HiSeq or NextSeq.
With further indices, several kits can also be combined for example to reach Y × 24 or Y x 48 samples.
One advantage of the MACE kit is that the samples are pooled after generating barcoded cDNAs. The 6x and 24x kits are designed to pool up to 6 and 24 libraries, respectively. The kit contains reactions to generate up to 6 or 24 libraries in the pre-pooling step and one post-pool reaction in the 6x kit and two post pool reactions in the 24x kit. For additional pools (e.g. for only two samples), additional post-pooling reagents can be purchased separately (cat.no. 16040.1 and 16040.2
Sequence your MACE -Seq libraries with other libraries
Since MACE-Sequencing does not require any specific sequencing primers but uses the standard primers for Illumina machines, you can easily sequence your MACE-Seq projects with other projects simultanteously.
We provide dual indices for secure identification of the samples.
MACE-Seq for IonTorrent
MACE-Seq is now also available for IonTorrent sequencers. Please ask for further information at firstname.lastname@example.org
Required input material: >5 ng totalRNA (can vary depending on tissue and quality of RNA)
Number of samples 1 – 96 (depending on Kit)
Time for sample preparation of 96 samples: 2 day
Please send us an e-mail at email@example.com or give us a call at: +49 69 95739710
Please contact us for a free trial kit at firstname.lastname@example.org
Customers in Japan: please contact FUNAKOSHI.
NEWS: Our kit received five Stars from “BIOZ“
We also offer MACE-Seq as a service including optional RNA extraction and bioinformatics.
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Tosches MA, Yamawaki TM, Naumann RK, Jacobi AA, Tushev G, Laurent G. Evolution of pallium, hippocampus, and cortical cell types revealed by single-cell transcriptomics in reptiles. Science. 2018 May 25;360(6391):881-888.
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Nold-Petry CA, Lo CY, Rudloff I, Elgass KD, Li S, Gantier MP, Lotz-Havla AS, Gersting SW, Cho SX, Lao JC, Ellisdon AM, Rotter B, Azam T, Mangan NE, Rossello FJ, Whisstock JC, Bufler P, Garlanda C, Mantovani A, Dinarello CA, Nold MF. IL-37 requires the receptors IL-18Ra and IL-1R8 (SIGIRR) to carry out its multifaceted anti-inflammatory program upon innate signal transduction.Nat Immunol. 2015 Mar 2
More references at: https://genxpro.net/publications/
The MACE-kit is for research use only.