The MACE-Seq Kit

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Our highly successful MACE-Seq service is now also available as a kit. It contains all ingredients to perform MACE-Seq. In contrast to other “3’mRNA Seq”  or “Tag-Seq” approaches the MACE-Seq Kit includes our patented TrueQuant method (molecular barcodes) to avoid PCR-bias, for the generation of qualitative and quantitative accurate data on any Illumina sequencing machine. MACE-Seq allows for accurate end cost efficient high-resolution gene expression profiling, genotyping in transcripts, allele frequency determination and the analysis of alternative polyadenylation. Up to 96 samples can be processed in parallel.

Bioinformatics

The MACE kit comes with bioinformatics-vouchers for a convenient analysis of the data on our servers.  As MACE data have some specificities, we have developed an optimized analysis pipeline that you can access using the vouchers. Simply upload your data using the provided voucher-codes to our servers. We provide complete result tables compatible with Excel, as well as plots of your data and Gene Ontology enrichment analyses. The raw and the refined data is accessible via our easily understandable web interface at http://tools.genxpro.net.

Further bioinformatics analyses are possible e.g. SNP-analysis, alternative polyadenylation or allele frequency determination (see Bioinformatics)

Therefore you do not need bioinformatians nor special hardware for the detailed analysis of the complex data.

Analyse 6 samples simultaneously on the MiSeq or 96 samples on a HiSeq or NextSeq500

The kit is available as 6x MACE version for up to 6 MACE libraries on the Illumina MiSeq platform and a 24x version for the Illumina HiSeq or NextSeq.

With further indices, several kits can also be combined for example to reach 4×24 samples.

Datasheet:

Required input material:  >10 ng totalRNA (can vary depending on tissue and quality of RNA)

Number of samples 1 – 96 (depending on Kit)

Time for sample preparation of  96 samples: 2 days

Prices:

Kit for MiSeq (Illumina), Single End Sequencing

6x Kit:                                                             390.00 EUR (cat. no. 16046.1)

Bioinformatics voucher 6x:                        360.00 EUR (cat. no.16046.2)

Kit for HiSeq or NextSeq (Illumina), Single End Sequencing

24x Kit:                                                           1,320.00 EUR (cat. no.160424.1)

2x Additional Post-Pool reactions1:                80.00 EUR (cat.no. 16040.1)

4x Additional Post-Pool reactions1:              140.00 EUR (cat.no. 16040.2)

Bioinformatics voucher 24x*:                    1,080.00 EUR (cat. no.160424.2)

Additional adapter set to combine up to four kits: 160.00 EUR (cat. no.160424.3)

(All prices without tax)

*the bioinformatics is limited to model organisms and includes annotation, quantification, differential gene expression (DE) analyses of pairwise comparisons (p-values for DE, log 2 Fold Changes) and GO enrichment analyses in our MACE cloud server. Data is accessible as downloadable .xls table and through our web interface at http://tools.genxpro.net

1Pooling strategy: One advantage of the MACE kit is that the samples are pooled after generating barcoded cDNAs. The 6x and 24x kits are designed to pool up to 6 and 24 libraries, respectively. The kit contains reactions to generate up to 6 or 24 libraries in the pre-pooling step and one post-pool reaction in the 6x kit and two post pool reactions in the 24x kit. For pools below 6 samples (e.g. for only two samples), additional post-pooling reagents can be purchased separately.

Interested?

Please send us an e-mail at info@genxpro.de or give us a call at: +49 69 95739710

Free Trial!

Please contact us for a free trial kit at info@genxpro.de

Customers in Japan: please contact FUNAKOSHI.

NEWS: Our kit received five Stars from “BIOZ

Service

We also offer MACE-Seq as a service including optional RNA extraction and bioinformatics.

References:

Tushev G, Glock C, Heumüller M, Biever A, Jovanovic M, Schuman EM.Alternative 3′ UTRs Modify the Localization, Regulatory Potential, Stability, and Plasticity of mRNAs in Neuronal Compartments.Neuron. 2018 May 2;98(3):495-511

Tosches MA, Yamawaki TM, Naumann RK, Jacobi AA, Tushev G, Laurent G. Evolution of pallium, hippocampus, and cortical cell types revealed by single-cell transcriptomics in reptiles. Science. 2018 May 25;360(6391):881-888.

Agarwal R, Cao Y, Hoffmeier K, Krezdorn N, Jost L, Meisel AR, Jüngling R, Dituri F, Mancarella S, Rotter B, Winter P, Giannelli G.Precision medicine for hepatocelluar carcinoma using molecular pattern diagnostics: results from a preclinical pilot study. Cell Death Dis. 2017 Jun 8;8(6):e2867.

Casado-Díaz A, Anter J, Müller S, Winter P, Quesada-Gómez JM, Dorado G. Transcriptomic Analyses of Adipocyte Differentiation From Human Mesenchymal Stromal-Cells (MSC). J Cell Physiol. 2016 Jun 28

Hofer TP, Zawada AM, Frankenberger M, Skokann K, Satzl AA, Gesierich W,
Schuberth M, Levin J, Danek A, Rotter B, Heine GH, Ziegler-Heitbrock L. slan-defined subsets of CD16-positive monocytes:Characterization of subsets of the CD16-positive monocytes: impact of granulomatous inflammation and M-CSF-receptor mutation. Blood. 2015 Oct 6. pii: blood-2015-06-651331.

Nold-Petry CA, Lo CY, Rudloff I, Elgass KD, Li S, Gantier MP, Lotz-Havla AS, Gersting SW, Cho SX, Lao JC, Ellisdon AM, Rotter B, Azam T, Mangan NE, Rossello FJ, Whisstock JC, Bufler P, Garlanda C, Mantovani A, Dinarello CA, Nold MF. IL-37 requires the receptors IL-18Ra and IL-1R8 (SIGIRR) to carry out its multifaceted anti-inflammatory program upon innate signal transduction.Nat Immunol. 2015 Mar 2

More references at: https://genxpro.net/publications/ 

 

Disclaimer:

The MACE-kit is for research use only.

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