TrueQuant SmallRNA Seq Kit for Ultra Low Input, GEL FREE, Single Tube Protocol, UMIs & UDIs
The GenXPro small RNA sequencing kit (v1.0) can be used to prepare small RNA libraries to be sequenced on Illumina® NGS instruments starting with 0.01 to 500 ng of total RNA.
The library preparation methodology used in this kit allows an accurate identification and quantification of microRNAs, piRNAs and other small RNAs.
Fast & simple single Tube Protocol, Gel free
The entire workflow takes place in a single tube. This minimizes losses and increases the efficiency tremendously. Even 0.01 ng of total RNA can be sufficient input material for library preparation.
Less PCR cycles, less duplicates – more output
Thanks to the highly efficient method to generate a sequencable library from small RNA, usually between 5 and 14 PCR cycles less are needed to obtain the minimum amount needed to load a sequencing lane compared to other standard methods. Our kit therefore often allows to drastically reduce the sequencing costs of smallRNA between 50% and 80% compared to most other methods.
Automation
The single tube protocol strongly facilitates highly paralleled analyses of many samples on liquid handling systems.
TrueQuant Technology
GenXPro small RNA sequencing kit applies the proprietary TrueQuant Technology that eliminates PCR-derived copies from the generated gene expression data by th use of uniqe molecular identifiers (UMI)*. Following sequencing of the adaptor-ligated and PCR-amplified libraries, only the sequence with highest quality of each TrueQuant adapter plus template-sequence combination are maintained.
*for licensing information for the use UMIs in Germany, please find more information here.
Liquid Biopsies from only 200 µl of Plasma
Our protocol allows to generate smallRNA-Seq profiles from down to 200 µl of plasma.
High Technical Reliability and Sensitivity
Scatterplots and pearson correlations (R) of comparisons between different amounts of totalRNA from the same sample.
Protocol for low input amounts, even from strongly degraded total RNA
Because of the single tube protocol that avoids gel purification steps, combined with the TrueQuant method (Unique Molecular Identifiers, UMI, patented), very low amounts of input material (0.01 ng) even from strongly degraded total RNA is suitable for processing.
Principle and Procedure
Starting with DNase-treated and quality-controlled total RNA, adapters containing TrueQuant barcodes are ligated to both ends of the smallRNA, reverse transcribed and the final libraries are ready for sequencing on any of the Illumina MiniSeq, MiSeq, HiSeq, and NextSeq platforms. The present kit allows for multiplexing of up to 96 samples.
Trial possible
Please send us an e-mail (info@genxpro.de) if you are interested in testing our smallRNA-kit.
Service
We also offer regular and ultra-low input smallRNA seq service.
Catalog numbers
Kit for MiSeq, HiSeq or NextSeq (Illumina), Single End Sequencing
| Name | Cat. Nr. |
| TQ-SmallRNA-Seq-6x | 16047.8 |
| TQ-SmallRNA-Seq-24x | 16047.24 |
| TQ-SmallRNA-Seq-48x | 16047.48 |
| TQ-SmallRNA-Seq-96x | 16047.96 |
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Orders or Quotations
please use our web-tool at https://tools.genxpro.net/quotation or contact us at info@genxpro.de.
References
Lipps, C.; Northe, P.; Figueiredo, R.; Rohde, M.; Brahmer, A.; Krämer-Albers, E.-M.; Liebetrau, C.; Wiedenroth, C.B.; Mayer, E.; Kriechbaum, S.D.; Dörr, O.; Nef, H.; Hamm, C.W.; Keller, T.; Troidl, C. Non-Invasive Approach for Evaluation of Pulmonary Hypertension Using Extracellular Vesicle-Associated Small Non-Coding RNA. Biomolecules2019, 9, 666. https://doi.org/10.3390/biom9110666
Wecker T, Hoffmeier K, Plötner A, Grüning BA, Horres R, Backofen R, Reinhard T, Schlunck G.MicroRNA Profiling in Aqueous Humor of Individual Human Eyes by Next-Generation Sequencing.Invest Ophthalmol Vis Sci. 2016 Apr 1;57(4):1706-1713.
Müller S, Raulefs S, Bruns P, Afonso-Grunz F, Plötner A, Thermann R, Jäger C, Schlitter AM, Kong B, Regel I, Roth WK, Rotter B, Hoffmeier K, Kahl G, Koch I, Theis FJ, Kleeff J, Winter P, Michalski CW. Next-generation sequencing reveals novel differentially regulated mRNAs, lncRNAs, miRNAs, sdRNAs and a piRNA in pancreatic cancer. Mol Cancer. 2015 Apr 25;14(1):94.
Further References (link to Pubmed)
