TrueQuant: recognize PCR-bias and eliminate it!
In the year 2008 we have invented the „TrueQuant“ method which later became also known as „Molecular Barcoding“ or the use of „Unique Molecular Identifiers“ (UMI), “SafeSeq” or “DigitalSequencing(TM)”.
With TrueQuant, we label PCR-template molecules prior to their amplification, such that each molecule consists of a unique sequence. This is achieved by incorporating a poly-N nucleotide (the TrueQuant Adapter or “UMI”) to the templates. Ideally, each template molecule can then be identified by its unique combination of the TrueQuant sequence and the template sequence. After PCR amplification, PCR copies can be identified and eliminated from the dataset and hence uneven amplification and artifacts generated during the PCR can be almost completely eliminated.
This is especially important for diagnostics as well as for methods that imply low amounts of starting material, such as in single cell sequencing, laser capture microdissection (LCM) or from liquid and other biopsies including NIPT.
TrueQuant is highly recommended for the analysis of nucleotide populations containing numerous similar sequences, such as smallRNAs, ChIP-Seq tags, Aptamers, RAD-Seq tags or GBS-tags.
If you would like to use Unique Molecular Barcodes in Germany, please buy our NGS kits oder ask for a license under firstname.lastname@example.org
The use of unique barcodes for single molecules (unique molecular identifiers, molecular barcodes) is protected by us for numerous applications under the patent number 102008025656. Users of the method in Germany, be it via the use of kits that have implemented the method or by other means might therefore infringe our patent.
Please send us an e-mail if you are unsecure about the patent situation when using UMIs in Germany. We offer fair licensing solutions to public institutes and companies for using UMIs for research or diagnostics.
TrueQuant Adapter Kits
TrueQuant adapters can now also be purchased for different applications, including sCHiP-seq, Atac-Seq, RAD-Seq / GBS, MethylSeq and many more. The technique is also implemented in our other NGS-Kits.
Please contact us at email@example.com