panRNA-Seq

TrueQuant panRNA-Seq – Discover More of the Transcriptome

GenXPro’s TQ panRNA-Seq is a next-generation total RNA-Seq technology that reveals the full complexity of RNA populations.

Compared to conventional small RNA-Seq, TQ panRNA-Seq uncovers up to ten times more different transcript species, providing a far more complete picture of RNA expression.

pan-RNA-Sequencing

Key Advantages

  • Comprehensive RNA Discovery
    panRNA-Seq detects the entire spectrum of RNA molecules
    from small RNAs to longer non-coding RNAs (ncRNAs) that standard small RNA-Seq often misses.
  • Superior Coverage of Long ncRNAs and piRNAs
    Our method excels in identifying longer ncRNAs, including piRNAs, which play important roles in gene regulation, development, and disease. This means more biologically relevant information for your research.
  • Optimized for EVs and Cell-Free RNA
    Specifically developed for challenging samples such as extracellular vesicles and cell-free RNA, pan-RNA-Seq delivers reliable, highly sensitive results where other methods fail to capture the transcriptome’s diversity.

RNA-Seq EVs

Illustration of EVs

Conventional vs. pan-RNA Sequencing

TQ panRNA-Seq vs. small-RNA Seq

Small RNA from 5 different plasma samples was sequenced using GenXPro TQ pan-RNA-Seq and small RNA-Seq library preparation method, sequencing with 1x 75 bps, Illumina.

Sequencing depth: For the calculation of the intra-sample coefficient of variation, another dataset was used with only 3.5 M reads.

Comparison sample reads pan-RNA-Seq vs small-RNA-Seq

Number of different transcript-species identified with small RNA-Seq and TQ pan-RNA-Seq after filtering all transcript species

Number of different transcript-species identified with small RNA-Seq and TQ panRNA-Seq after filtering

Conclusion

Our TQ pan-RNA-Seq method empowers you to gain deeper insights into RNA biology – whether for biomarker discovery, diagnostics, or fundamental research.

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