SARS-CoV-2 VoC qPCR Assay N501Y del69-70
Simultaneous SARS-CoV-2 & VoC-PCR for Variants of Concern B.1.1.7, B.1.351 and P1
Besides the M-gene, our assay specifically detects the N501Y mutation present in all VoC (B.1.1.7; B.1.351 and P1). In addition, the assay is specific for the S gene (wt) and detects the del69-70 by drop-out. This way, our assay detects both wt and VoC via at least two targets (dal target assay).
Our assay also includes RNaseP as sampling control. The assay can be performed on common qPCR instruments with detection channels of FAM, Joe, Cy5, and ROX.
Only one qPCR reaction is needed for fastest results.
The assay is for research use only at the moment. CE-IVD is in progress.
Left: WT leading to a S-gene signal but no signal with the N501Y specific assay. Right: B220.127.116.11 strain with an N501Y specific signal but no signal for the S-gene because of D69/70 (drop out). Both strains lead to speficic PCR product signals for the SARS-CoV-2 M gene and the RP-gene (human control gene).
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